Beebe-Wang, Nicasia2019-03-262017-052017-07-142017http://nrs.harvard.edu/urn-3:HUL.InstRepos:38811503Promoters play a key role in gene regulation. Although progress has been made to understand the elements which make up a promoter, the identification of all of the regulatory elements which comprise the promoter remains challenging due to the high variability of promoter sequences. In this thesis, I aim to identify regulatory elements in promoter regions using deep learning. Specifically, I employ a convolutional neural network (CNN) to predict whether a given genomic sequence contains a promoter versus several null models, i.e. background sequences. I compare the performance of the CNN model for each null model and perform saliency analysis to visualize what the network has learned. The main result I found is that the null model must be carefully selected to avoid learning confounding signals such as nucleotide biases. I found that a dinucleotide shuffle of transcription start sites was able to find known regulatory elements associated with bi-directional promoters.application/pdfenComputer ScienceThesis or Dissertation2019-03-260000-0001-7031-2665