Silva, LaurieLoregian, AriannaPari, GregoryStrang, BlairCoen, Donald2019-10-052010Silva, L. A., A. Loregian, G. S. Pari, B. L. Strang, and D. M. Coen. 2010. “The Carboxy-Terminal Segment of the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 Is Crucial for Viral Replication.” Journal of Virology 84 (21): 11563–68. https://doi.org/10.1128/jvi.01033-10.0022-538X1070-63211098-5514http://nrs.harvard.edu/urn-3:HUL.InstRepos:41482950The amino-terminal 290 residues of UL44, the presumed processivity factor of human cytomegalovirus DNA polymerase, possess all of the established biochemical activities of the full-length protein, while the carboxyterminal 143 residues contain a nuclear localization signal (NLS). We found that although the amino-terminal domain was sufficient for origin-dependent synthesis in a transient-transfection assay, the carboxy-terminal segment was crucial for virus replication and for the formation of DNA replication compartments in infected cells, even when this segment was replaced with a simian virus 40 NLS that ensured nuclear localization. Our results suggest a role for this segment in viral DNA synthesis.en-USJournal Article2019-10-0510.1128/JVI.01033-10