Wang, ZhenZhang, HongShen, Xu-HuiJin, Kui-LiYe, Guo-fenQian, LiLi, BoZhang, Yong-HongShi, Guo-PingSong, Yiqing2019-10-142011Wang, Zhen, Hong Zhang, Xu-Hui Shen, Kui-Li Jin, Guo-fen Ye, Li Qian, Bo Li, Yong-Hong Zhang, and Guo-Ping Shi. 2011. “Immunoglobulin E and Mast Cell Proteases Are Potential Risk Factors of Human Pre-Diabetes and Diabetes Mellitus.” Edited by Yiqing Song. PLoS ONE 6 (12): e28962. doi:10.1371/journal.pone.0028962.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:41543162Background: Recent studies have suggested that mast-cell activation and inflammation are important in obesity and diabetes. Plasma levels of mast cell proteases and the mast cell activator immunoglobulin E (IgE) may serve as novel inflammatory markers that associate with the risk of pre-diabetes and diabetes mellitus. Methods and Results: A total of 340 subjects 55 to 75 years of age were grouped according to the American Diabetes Association 2003 criteria of normal glucose tolerance, pre-diabetes, and diabetes mellitus. The Kruskal-Wallis test demonstrated significant differences in plasma IgE levels (P = 0.008) among groups with different glucose tolerance status. Linear regression analysis revealed significant correlations between plasma levels of chymase (P = 0.030) or IgE (P = 0.022) and diabetes mellitus. Ordinal logistic regression analysis showed that IgE was a significant risk factor of pre-diabetes and diabetes mellitus (odds ratio [OR]: 1.674, P = 0.034). After adjustment for common diabetes risk factors, including age, sex, hypertension, body-mass index, cholesterol, homeostatic model assessment (HOMA) index, high-sensitivity C-reactive protein (hs-CRP), and mast cell chymase and tryptase, IgE remained a significant risk factor (OR: 1.866, P = 0.015). Two-variable ordinal logistic analysis indicated that interactions between hs-CRP and IgE, or between IgE and chymase, increased further the risks of developing pre-diabetes and diabetes mellitus before (OR: 2.204, P = 0.044; OR: 2.479, P = 0.033) and after (OR: 2.251, P = 0.040; OR: 2.594, P = 0.026) adjustment for common diabetes risk factors. Conclusions: Both IgE and chymase associate with diabetes status. While IgE and hs-CRP are individual risk factors of prediabetes and diabetes mellitus, interactions of IgE with hs-CRP or with chymase further increased the risk of pre-diabetes and diabetes mellitus.en-USJournal Article2019-10-1410.1371/journal.pone.0028962