Ostafe, RalucaProdanovic, RadivojeUng, W. LloydWeitz, David A.Fischer, Rainer2019-10-092014Ostafe, Raluca, Radivoje Prodanovic, W. Lloyd Ung, David A. Weitz, and Rainer Fischer. 2014. “A High-Throughput Cellulase Screening System Based on Droplet Microfluidics.” Biomicrofluidics8 (4): 041102. https://doi.org/10.1063/1.4886771.1932-1058http://nrs.harvard.edu/urn-3:HUL.InstRepos:41511278A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.en-USJournal Article2019-10-0910.1063/1.4886771