Browsing by Author "Langenau, David"

Now showing items 1-14 of 14

    • Cross-Species Array Comparative Genomic Hybridization Identifies Novel Oncogenic Events in Zebrafish and Human Embryonal Rhabdomyosarcoma 

      Chen, Eleanor Y.; Dobrinski, Kimberly P.; Brown, Kim H.; Clagg, Ryan; Edelman, Elena; Ignatius, Myron S.; Chen, Jin Yun Helen; Brockmann, Jillian; Nielsen, G. Petur; Ramaswamy, Sridhar; Keller, Charles; Lee, Charles; Langenau, David M. (Public Library of Science, 2013)
      Human cancer genomes are highly complex, making it challenging to identify specific drivers of cancer growth, progression, and tumor maintenance. To bypass this obstacle, we have applied array comparative genomic hybridization ...

    • High-Throughput Cell Transplantation Establishes That Tumor-Initiating Cells Are Abundant in Zebrafish T-Cell Acute Lymphoblastic Leukemia 

      Smith, Alexandra C. H.; Raimondi, Aubrey R.; Salthouse, Chris D.; Ignatius, Myron S; Blackburn, Jessica S; Mizgirev, Igor V.; Storer, Narie Yoo; de Jong, Jill L. O.; Chen, Aye Tin Maung; Zhou, Yi; Revskoy, Sergei; Zon, Leonard Ira; Langenau, David M. (American Society of Hematology, 2010)
      Self-renewal is a feature of cancer and can be assessed by cell transplantation into immune-compromised or immune-matched animals. However, studies in zebrafish have been severely limited by lack of these reagents. Here, ...

    • The Histone Methyltransferase SUV39H1 Suppresses Embryonal Rhabdomyosarcoma Formation in Zebrafish 

      Albacker, Colleen Elizabeth; Storer, Narie Yoo; Langdon, Erin M.; DiBiase, Anthony; Zhou, Yi; Langenau, David M.; Zon, Leonard Ira (Public Library of Science, 2013)
      Epigenetics, or the reversible and heritable marks of gene regulation not including DNA sequence, encompasses chromatin modifications on both the DNA and histones and is as important as the DNA sequence itself. Chromatin-modifying ...

    • Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish 

      Tang, Qin; Moore, John C.; Ignatius, Myron S.; Tenente, Inês M.; Hayes, Madeline N.; Garcia, Elaine G.; Torres Yordán, Nora; Bourque, Caitlin; He, Shuning; Blackburn, Jessica S.; Look, A. Thomas; Houvras, Yariv; Langenau, David M. (Nature Publishing Group, 2016)
      Cancers contain a wide diversity of cell types that are defined by differentiation states, genetic mutations and altered epigenetic programmes that impart functional diversity to individual cells. Elevated tumour cell ...

    • Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs) 

      Moore, Finola Elizabeth; Reyon, Deepak; Sander, Jeffry D.; Martinez, Sarah A.; Blackburn, Jessica S.; Khayter, Cyd; Ramirez, Cherie Lynn; Joung, Jae Keith; Langenau, David M. (Public Library of Science, 2012)
      Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in ...

    • Interrogation of the RP-MDM2-P53 Axis in Human Ribosomopathies 

      Raiser, David Michael (2015-05-15)
      Ribosomopathies such as Diamond-Blackfan anemia (DBA) and 5q- syndrome are human diseases characterized by heterozygous loss or mutation of ribosome-associated genes. Hallmarks of these diseases include a macrocytic anemia ...

    • Lineage of origin in rhabdomyosarcoma informs pharmacological response 

      Abraham, Jacob; Nunez-Alvarez, Y.; Hettmer, S; Carrio, E.; Chen, Hung-I Harry; Nishijo, K.; Huang, Elaine; Prajapati, Suresh I.; Walker, Robert L.; Davis, Sean; Rebeles, Jennifer; Wiebush, Hunter; McCleish, Amanda T.; Hampton, Sheila T.; Bjornson, Christopher R.R.; Brack, Andrew Stephen; Wagers, Amy Jo; Rando, Thomas A.; Capecchi, Mario R.; Marini, Frank C.; Ehler, Benjamin R.; Zarzabal, Lee Ann; Goros, Martin W.; Michalek, Joel E.; Meltzer, Paul S.; Langenau, David M.; LeGallo, Robin D.; Mansoor, Asif Imran; Chen, Yidong; Suelves, Monica; Rubin, Brian P.; Keller, Charles (Cold Spring Harbor Laboratory Press, 2014)
      Lineage or cell of origin of cancers is often unknown and thus is not a consideration in therapeutic approaches. Alveolar rhabdomyosarcoma (aRMS) is an aggressive childhood cancer for which the cell of origin remains ...

    • Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma 

      Tenente, Inês M; Hayes, Madeline N; Ignatius, Myron S; McCarthy, Karin; Yohe, Marielle; Sindiri, Sivasish; Gryder, Berkley; Oliveira, Mariana L; Ramakrishnan, Ashwin; Tang, Qin; Chen, Eleanor Y; Petur Nielsen, G; Khan, Javed; Langenau, David M (eLife Sciences Publications, Ltd, 2017)
      Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely ...

    • Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency 

      Blackburn, Jessica S.; Liu, Sali; Raiser, Dave Michael; Martinez, Sarah A.; Feng, Hui; Meeker, Nathan D.; Gentry, Jeffery; Neuberg, Donna S.; Look, A. Thomas; Ramaswamy, Sridhar; Bernards, Andre; Trede, Nikolaus S.; Langenau, David M. (2012)
      NOTCH1 pathway activation contributes to the pathogenesis of over 60% of T-cell acute lymphoblastic leukemia (T-ALL). While Notch is thought to exert the majority of its effects through transcriptional activation of Myc, ...

    • Optimized cell transplantation using adult rag2 mutant zebrafish 

      Tang, Qin; Abdelfattah, Nouran S.; Blackburn, Jessica S.; Moore, John C.; Martinez, Sarah A.; Moore, Finola E.; Lobbardi, Riadh; Tenente, Inês M.; Ignatius, Myron S.; Berman, Jason N.; Liwski, Robert S.; Houvras, Yariv; Langenau, David M. (2014)
      Cell transplantation into adult zebrafish has lagged behind mouse due to the lack of immune compromised models. Here, we have created homozygous rag2E450fs mutant zebrafish that have reduced numbers of functional T and B ...

    • Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish 

      Moore, Finola E.; Garcia, Elaine G.; Lobbardi, Riadh; Jain, Esha; Tang, Qin; Moore, John C.; Cortes, Mauricio; Molodtsov, Aleksey; Kasheta, Melissa; Luo, Christina C.; Garcia, Amaris J.; Mylvaganam, Ravi; Yoder, Jeffrey A.; Blackburn, Jessica S.; Sadreyev, Ruslan I.; Ceol, Craig J.; North, Trista E.; Langenau, David M. (The Rockefeller University Press, 2016)
      Hematopoiesis culminates in the production of functionally heterogeneous blood cell types. In zebrafish, the lack of cell surface antibodies has compelled researchers to use fluorescent transgenic reporter lines to label ...

    • T-Lymphoblastic Lymphoma Cells Express High Levels of BCL2, S1P1, and ICAM1, Leading to a Blockade of Tumor Cell Intravasation 

      Feng, Hui; Stachura, David L.; White, Richard; Gutierrez, Alejandro; Zhang, Lu; Sanda, Takaomi; Jette, Cicely A.; Testa, Joseph R.; Neuberg, Donna S.; Langenau, David M.; Kutok, Jeffery Lorne; Zon, Leonard Ira; Traver, David; Fleming, Mark Daniel; Kanki, John P.; Look, A. Thomas (Elsevier BV, 2010)
      The molecular events underlying the progression of T-lymphoblastic lymphoma (T-LBL) to acute T-lymphoblastic leukemia (T-ALL) remain elusive. In our zebrafish model, concomitant overexpression of bcl-2 with Myc accelerated ...

    • Through the Looking Glass: Visualizing Leukemia Growth, Migration, and Engraftment Using Fluorescent Transgenic Zebrafish 

      Moore, Finola Elizabeth; Langenau, David M. (Hindawi Publishing Corporation, 2012)
      Zebrafish have emerged as a powerful model of development and cancer. Human, mouse, and zebrafish malignancies exhibit striking histopathologic and molecular similarities, underscoring the remarkable conservation of genetic ...

    • A Zebrafish Model of Uveal Melanoma 

      Rose, Kristin (2015-05-06)
      Uveal melanoma is the most common primary intraocular tumor in adults, and is often characterized by poor prognosis and few effective therapeutic options. The typical site of metastasis for uveal melanoma is the liver, and ...