Person: Velilla, Jose
Loading...
Email Address
AA Acceptance Date
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
Velilla
First Name
Jose
Name
Velilla, Jose
Search Results
Now showing 1 - 1 of 1
Publication Structural Basis of Colibactin Activation by the ClbP Peptidase(Springer Science and Business Media LLC, 2022-10-17) Velilla, Jose; Volpe, Matthew; Kenney, Grace; Walsh, Richard; Balskus, Emily; Gaudet, RachelleColibactin, a DNA crosslinking agent produced by gut bacteria, is implicated in colorectal cancer. Its biosynthesis uses a prodrug resistance mechanism: a non-toxic precursor assembled in the cytoplasm is activated after export to the periplasm. This activation is mediated by ClbP, an inner-membrane peptidase with an N-terminal periplasmic catalytic domain and a C-terminal three-helix transmembrane domain. Although the transmembrane domain is required for colibactin activation, its role in catalysis is unclear. Our structure of full-length ClbP bound to a product analog reveals an interdomain interface important for substrate binding and enzyme stability, and interactions that explain the selectivity of ClbP for the N-acyl-D-asparagine prodrug motif. Based on structural and biochemical evidence, we propose that ClbP dimerizes to form an extended substrate-binding site that can accommodate a pseudodimeric precolibactin, with its two terminal prodrug motifs in the two ClbP active sites, thus enabling the coordinated activation of both electrophilic warheads.