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Wu, Sarah

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Wu

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Sarah

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Wu, Sarah

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2018 - 20192

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Author's Publications: search.filters.isAuthorOfPublication.3d6f1387-59d7-44e6-844b-db0ff5a7bd9e

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    Wavelength-Encoded Laser Particles for Massively-Multiplexed Cell Tagging
    (Springer Nature, 2018-11-07) Martino, Nicola; Kwok, Sheldon; Forward, Sarah; Liapis, Andreas; Wu, Sarah; Dannenberg, Paul; Yun, Seok-Hyun; Jang, Hoon; Kim, Hwi-Min; Wu, Jiamin; Jang, Sun-Joo; Lee, Yong-Hee
    Large-scale single-cell analyses have become increasingly important given the role of cellular heterogeneity in complex biological systems. However, no current techniques enable optical imaging of uniquely-tagged individual cells. Fluorescence-based approaches can only distinguish a small number of distinct cells or cell groups at a time because of spectral crosstalk between conventional fluorophores. Here we investigate large-scale cell tracking using intracellular laser particles as imaging probes that emit coherent laser light with a characteristic wavelength. Made of silica-coated semiconductor microcavities, these laser particles have single-mode emission over a broad range from 1170 to 1580 nm with sub-nm linewidths, enabling massive spectral multiplexing. We explore the stability and biocompatibility of these probes in vitro and their utility for wavelength-multiplexed cell tagging and imaging. We demonstrate real-time tracking of thousands of individual cells in a 3D tumour model over several days showing different behavioural phenotypes.
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    Wavelength-Encoded Laser Particles for Massively Multiplexed Cell Tagging
    (Springer Science and Business Media LLC, 2019-07-22) Martino, Nicola; Kwok, Sheldon; Liapis, Andreas; Forward, Sarah; Jang, Hoon; Kim, Hwi-Min; Wu, Sarah; Wu, Jiamin; Dannenberg, Paul; Jang, Sun-Joo; Lee, Yong-Hee; Yun, Seok-Hyun
    Large-scale single-cell analyses have become increasingly important given the role of cellular heterogeneity in complex biological systems. However, no current techniques enable optical imaging of uniquely-tagged individual cells. Fluorescence-based approaches can only distinguish a handful of distinct cells or cell groups at a time because of spectral crosstalk between conventional fluorophores. Here we show a novel class of imaging probes emitting coherent laser light, called laser particles. Made of silica-coated semiconductor microcavities, these laser particles have single-mode emission over a broad range from 1170 to 1580 nm with sub-nm linewidths, enabling massive spectral multiplexing. We demonstrate the stability and biocompatibility of these probes in vitro and their utility for wavelength-multiplexed cell tagging and imaging. We demonstrate real-time tracking of thousands of individual cells in a 3D tumor model for several days showing different behavioral phenotypes. We expect laser particles will enable new approaches for single-cell analyses.