Person: Kolesky, David
Loading...
Email Address
AA Acceptance Date
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
Kolesky
First Name
David
Name
Kolesky, David
6 results
Search Results
Now showing 1 - 6 of 6
Publication Controlling Material Reactivity Using Architecture(Wiley-Blackwell, 2015) Sullivan, Kyle T.; Zhu, Cheng; Duoss, Eric B.; Gash, Alexander E.; Kolesky, David; Kuntz, Joshua D.; Lewis, Jennifer; Spadaccini, Christopher M.3D-printing methods are used to generate reactive material architectures. Several geometric parameters are observed to influence the resultant flame propagation velocity, indicating that the architecture can be utilized to control reactivity. Two different architectures, channels and hurdles, are generated, and thin films of thermite are deposited onto the surface. The architecture offers an additional route to control, at will, the energy release rate in reactive composite materials.Publication High-Throughput Printing via Microvascular Multinozzle Arrays(Wiley-Blackwell, 2012) Hansen, Christopher J.; Saksena, Rajat; Kolesky, David; Vericella, John J.; Kranz, Stephen J.; Muldowney, Gregory P.; Christensen, Kenneth T.; Lewis, JenniferMicrovascular multinozzle arrays are designed and fabricated for high-throughput printing of functional materials. Ink-flow uniformity within these multigeneration, bifurcating microchannel arrays is characterized by computer modeling and microscopic particle image velocimetry (micro-PIV) measurements. Both single and dual multinozzle printheads are produced to enable rapid printing of multilayered periodic structures over large areas (≈1 m2).Publication 3D Bioprinting of Vascularized, Heterogeneous Cell-Laden Tissue Constructs(Wiley-Blackwell, 2014) Kolesky, David; Truby, Ryan; Gladman, Amelia Sydney; Busbee, Travis Alexander; Homan, Kimberly; Lewis, JenniferA new bioprinting method is reported for fabricating 3D tissue constructs replete with vasculature, multiple types of cells, and extracellular matrix. These intricate, heterogeneous structures are created by precisely co-printing multiple materials, known as bioinks, in three dimensions. These 3D micro-engineered environments open new avenues for drug screening and fundamental studies of wound healing, angiogenesis, and stem-cell niches.Publication Embedded 3D Printing of Strain Sensors within Highly Stretchable Elastomers(Wiley-Blackwell, 2014) Muth, Joseph Thomas; Vogt, Daniel; Truby, Ryan; Mengüç, Yiğit; Kolesky, David; Wood, Robert; Lewis, JenniferA new method, embedded-3D printing (e-3DP), is reported for fabricating strain sensors within highly conformal and extensible elastomeric matrices. e-3DP allows soft sensors to be created in nearly arbitrary planar and 3D motifs in a highly programmable and seamless manner. Several embodiments are demonstrated and sensor performance is characterized.Publication Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips(Nature Publishing Group, 2016) Homan, Kimberly; Kolesky, David; Skylar-Scott, Mark; Herrmann, Jessica; Obuobi, Humphrey; Moisan, Annie; Lewis, JenniferThree-dimensional models of kidney tissue that recapitulate human responses are needed for drug screening, disease modeling, and, ultimately, kidney organ engineering. Here, we report a bioprinting method for creating 3D human renal proximal tubules in vitro that are fully embedded within an extracellular matrix and housed in perfusable tissue chips, allowing them to be maintained for greater than two months. Their convoluted tubular architecture is circumscribed by proximal tubule epithelial cells and actively perfused through the open lumen. These engineered 3D proximal tubules on chip exhibit significantly enhanced epithelial morphology and functional properties relative to the same cells grown on 2D controls with or without perfusion. Upon introducing the nephrotoxin, Cyclosporine A, the epithelial barrier is disrupted in a dose-dependent manner. Our bioprinting method provides a new route for programmably fabricating advanced human kidney tissue models on demand.Publication 3D Bioprinting of Vascularized Human Tissues(2016-04-26) Kolesky, David; Lewis, Jennifer A.; Mooney, David; Joshi, Neel; Suo, ZhigangThe ability to manufacture human tissues that replicate the spatial, mechano-chemical, and temporal aspects of biological tissues would enable myriad applications, including drug screening, disease modeling, and tissue repair and regeneration. However, given the complexity of human tissues, this is a daunting challenge. Current biofabrication methods are unable to fully recapitulate the form and function of human tissues, which are composed of multiple cell types, extracellular matrices, and pervasive vasculature. My Ph.D. thesis focuses on advancing the capabilities of human tissue fabrication. Specifically, we demonstrate a multimaterial bioprinting method capable of producing 1D, 2D, and 3D vascularized tissue constructs by co-printing ECM, cell-laden, and fugitive inks. After these heterogeneous tissue constructs are printed and infilled with ECM, they are cooled to 4˚C to remove the fugitive ink leaving behind a pervasive network that is subsequently lined with endothelial cells. These constructs are ~ 1 mm in thickness and can be sustained for up to 14 days via rocking-based flow through their vasculature. We then created a new extracellular matrix that enabled the fabrication of tissues that exceed 1 cm in thickness that are perfused on a microfluidic chip for long time periods (> 6 weeks). To demonstrate functionality, growth factors are perfused via the vasculature to differentiate stem cells toward an osteogenic lineage in situ. Finally, we created renal proximal tubules (a sub-unit of kidney tissue) by this approach. Specifically, we constructed 3D tubules circumscribed by renal proximal tubule epithelial cells (PTECs). The PTECs form confluent, leak-tight epithelial monolayers that exhibit primary cilia and expresses Na+/K+ ATPase, Aquaporin 1, and K-cadherin. The combination of 3D geometry and on-chip perfusable nature gives rise to enhanced, polarized PTEC phenotypes that develop an enhanced brush border, basement membrane protein deposition, basolateral interdigitations, enhanced cell height, megalin expression, and albumin uptake relative to 2D controls. In summary, this multimaterial 3D bioprinting platform enables production of engineered human tissue constructs in which multiple cell types and vasculature are programmably placed within extracellular matrices. These 3D tissues may find potential applications in drug screening, disease models, and ultimately, tissue engineering and regenerative medicine.