Person: Weil, Ana
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Weil, Ana
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Publication Fecal Microbiota Transplant: Benefits and Risks(Oxford University Press, 2015) Weil, Ana; Hohmann, ElizabethPublication Vibrio cholerae genomic diversity within and between patients(Microbiology Society, 2017) Levade, Inès; Terrat, Yves; Leducq, Jean-Baptiste; Weil, Ana; Mayo-Smith, Leslie M.; Chowdhury, Fahima; Khan, Ashraful I.; Boncy, Jacques; Buteau, Josiane; Ivers, Louise C.; Ryan, Edward; Charles, Richelle; Calderwood, Stephen; Qadri, Firdausi; Harris, Jason; Larocque, Regina; Shapiro, B. JesseCholera is a severe, water-borne diarrhoeal disease caused by toxin-producing strains of the bacterium Vibrio cholerae. Comparative genomics has revealed ‘waves’ of cholera transmission and evolution, in which clones are successively replaced over decades and centuries. However, the extent of V. cholerae genetic diversity within an epidemic or even within an individual patient is poorly understood. Here, we characterized V. cholerae genomic diversity at a micro-epidemiological level within and between individual patients from Bangladesh and Haiti. To capture within-patient diversity, we isolated multiple (8 to 20) V. cholerae colonies from each of eight patients, sequenced their genomes and identified point mutations and gene gain/loss events. We found limited but detectable diversity at the level of point mutations within hosts (zero to three single nucleotide variants within each patient), and comparatively higher gene content variation within hosts (at least one gain/loss event per patient, and up to 103 events in one patient). Much of the gene content variation appeared to be due to gain and loss of phage and plasmids within the V. cholerae population, with occasional exchanges between V. cholerae and other members of the gut microbiota. We also show that certain intra-host variants have phenotypic consequences. For example, the acquisition of a Bacteroides plasmid and non-synonymous mutations in a sensor histidine kinase gene both reduced biofilm formation, an important trait for environmental survival. Together, our results show that V. cholerae is measurably evolving within patients, with possible implications for disease outcomes and transmission dynamics.Publication Ceftaroline Desensitization Procedure in a Pregnant Patient With Multiple Drug Allergies(Oxford University Press, 2015) Kuhlen, James L.; Blumenthal, Kimberly; Sokol, Caroline; Balekian, Diana S.; Weil, Ana; Varughese, Christy A.; Seiguer Shenoy, Erica; Banerji, AleenaValidated skin testing is lacking for many drugs, including ceftaroline. The cross-reactivity between ceftaroline and other β-lactam antibiotics is unknown. We report a case of a pregnant patient with cystic fibrosis and multiple drug allergies who required ceftaroline for methicillin-resistant Staphylococcus aureus pneumonia and underwent an uncomplicated empiric desensitization procedure.Publication Treatment Outcomes and Adverse Drug Reactions Associated with Ceftaroline Use(Oxford University Press, 2015) Blumenthal, Kimberly; Kuhlen, James; Weil, Ana; Varughese, Christy; Kubiak, David W.; Banerji, Aleena; Seiguer Shenoy, Erica