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Ahn, Seungkuk

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Ahn

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Seungkuk

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Ahn, Seungkuk

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2016 - 20182

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Author's Publications: search.filters.isAuthorOfPublication.54a10afc-e35b-4f5b-ab67-5ee5b8a07bd7

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    Formation of Multi-Component Extracellular Matrix Protein Fibers
    (Nature Publishing Group UK, 2018) Ahn, Seungkuk; Lee, Keel Yong; Parker, Kevin; Shin, Kwanwoo
    The extracellular matrix (ECM) consists of polymerized protein monomers that form a unique fibrous network providing stability and structural support to surrounding cells. We harnessed the fibrillogenesis mechanisms of naturally occurring ECM proteins to produce artificial fibers with a heterogeneous protein makeup. Using ECM proteins as fibril building blocks, we created uniquely structured multi-component ECM fibers. Sequential incubation of fibronectin (FN) and laminin (LAM) resulted in self-assembly into locally stacked fibers. In contrast, simultaneous incubation of FN with LAM or collagen (COL) produced molecularly stacked multi-component fibers because both proteins share a similar assembly mechanism or possess binding domains specific to each other. Sequential incubation of COL on FN fibers resulted in fibers with sandwiched layers because COL molecules bind to the external surface of FN fibers. By choosing proteins for incubation according to the interplay of their fibrillogenesis mechanisms and their binding domains (exposed when they unfold), we were able to create ECM protein fibers that have never before been observed.
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    Phototactic guidance of a tissue-engineered soft-robotic ray
    (American Association for the Advancement of Science (AAAS), 2016) Park, Sung-Jin; Gazzola, Mattia; Park, Kyung; Park, Shirley; Di Santo, Valentina; Blevins, Erin; Lind, Johan; Campbell, Patrick; Dauth, Stephanie; Capulli, Andrew; Pasqualini, Francesco; Ahn, Seungkuk; Cho, Alexander; Yuan, Hongyan; Maoz, Ben; Vijaykumar, Ragu; Choi, Jeong-Woo; Deisseroth, Karl; Lauder, George; Mahadevan, Lakshminarayanan; Parker, Kevin
    Inspired by the relatively simple morphological blueprint provided by batoid fish such as stingrays and skates, we create a biohybrid system that enables an artificial animal, a tissue-engineered ray, to swim and phototactically follow a light cue. By patterning dissociated rat cardiac myocytes on an elastomeric body enclosing a microfabricated gold skeleton, we replicated fish morphology at one-tenth scale and captured basic fin deflection patterns of batoid fish. Optogenetics allows for phototactic guidance, steering and turning maneuvers. Optical stimulation induced sequential muscle activation via serpentine patterned muscle circuits leading to coordinated undulatory swimming. The speed and direction of the ray was controlled by modulating light frequency and by independently eliciting right and left fins, allowing the biohybrid machine to maneuver through an obstacle course.