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Fonseka, Chamith

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Fonseka

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Chamith

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Fonseka, Chamith
Author's Publications: search.filters.isAuthorOfPublication.647dfea7-2d09-49ac-8b0b-691fcd4de1c6

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    Abnormal Dosage of Ultraconserved Elements Is Highly Disfavored in Healthy Cells but Not Cancer Cells
    (Public Library of Science, 2014) McCole, Ruth; Fonseka, Chamith; Koren, Amnon; Wu, C.-ting
    Ultraconserved elements (UCEs) are strongly depleted from segmental duplications and copy number variations (CNVs) in the human genome, suggesting that deletion or duplication of a UCE can be deleterious to the mammalian cell. Here we address the process by which CNVs become depleted of UCEs. We begin by showing that depletion for UCEs characterizes the most recent large-scale human CNV datasets and then find that even newly formed de novo CNVs, which have passed through meiosis at most once, are significantly depleted for UCEs. In striking contrast, CNVs arising specifically in cancer cells are, as a rule, not depleted for UCEs and can even become significantly enriched. This observation raises the possibility that CNVs that arise somatically and are relatively newly formed are less likely to have established a CNV profile that is depleted for UCEs. Alternatively, lack of depletion for UCEs from cancer CNVs may reflect the diseased state. In support of this latter explanation, somatic CNVs that are not associated with disease are depleted for UCEs. Finally, we show that it is possible to observe the CNVs of induced pluripotent stem (iPS) cells become depleted of UCEs over time, suggesting that depletion may be established through selection against UCE-disrupting CNVs without the requirement for meiotic divisions.
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    Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes
    (Nature Pub. Group, 2015) Beliveau, Brian; Boettiger, Alistair; Avendaño, Maier S.; Jungmann, Ralf; McCole, Ruth; Joyce, Eric F.; Kim-Kiselak, Caroline; Bantignies, Frédéric; Fonseka, Chamith; Erceg, Jelena; Hannan, Mohammed; Hoang, Hien G.; Colognori, David; Lee, Jeannie; Shih, William; Yin, Peng; Zhuang, Xiaowei; Wu, Chao-ting
    Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis.