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Song, Li

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    TRUST4: Immune Repertoire Reconstruction From Bulk and Single-Cell RNA-Seq Data
    (Springer Science and Business Media LLC, 2021-06) Song, Li; Cohen, David; Ouyang, Zhangyi; Cao, Yang; Hu, Xihao; Liu, Xiaole
    We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms.