Person: Kavanagh, Daniel Garrett
Email Address
AA Acceptance Date
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
First Name
Name
Search Results
Publication P16-08. Combined blockade of the PD-1 and IL-10 pathways synergistically enhance HIV-specific CD4 T cell functions
(BioMed Central, 2009) Porichis, Filippos; Kwon, DS; Tighe, DP; Pavlik, DF; Kavanagh, Daniel Garrett; Freeman, Gordon; Walker, Bruce; Kaufmann, Daniel E.Publication PD-1, IL-10, IFN-γ and IL-12 Form a Network to Regulate HIV-1-Specific CD4 T Cell and Antigen-Presenting Cell Function
(BioMed Central, 2012) Porichis, Filippos; Barblu, Lucie; Kwon, Douglas; Hart, M; Zupkosky, J; Freeman, Gordon; Kavanagh, Daniel Garrett; Kaufmann, Daniel E.Publication High throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry
(2014) Porichis, Filippos; Hart, Meghan G.; Griesbeck, Morgane; Everett, Holly L.; Hassan, Muska; Baxter, Amy E.; Lindqvist, Madelene; Miller, Sara M.; Soghoian, Damien Z.; Kavanagh, Daniel Garrett; Reynolds, Susan; Norris, Brett; Mordecai, Scott K.; Nguyen, Quan; Lai, Chunfai; Kaufmann, Daniel E.Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human leukocytes. This technique can be multiplexed and combined with fluorescent antibody protein staining to addressa variety of questions in heterogeneous cell populations. We demonstrate antigen-specific upregulation of IFNγ and IL-2 mRNAs in HIV- and CMV-specific T cells. We show simultaneous detection of cytokine mRNA and corresponding protein in single cells. We apply this method to detect mRNAs for which flow antibodies against the corresponding proteins are poor or are not available. We use this technique to show modulation of a microRNA critical for T cell function, miR-155. We adapt this assay for simultaneous detection of mRNA and proteins by Image Stream technology.