Person:
Saper, Clifford

Profile Picture

Email Address

AA Acceptance Date

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Saper

First Name

Clifford

Name

Saper, Clifford
Author's Publications: search.filters.isAuthorOfPublication.76e3422f-336b-4ff0-83c7-82b03bece581

Search Results

Now showing 1 - 7 of 7
  • Thumbnail Image
    Publication
    Armodafinil-induced wakefulness in animals with ventrolateral preoptic lesions
    (Dove Medical Press, 2014) Vetrivelan, Ramalingam; Saper, Clifford; Fuller, Patrick
    Armodafinil is the pharmacologically active R-enantiomer of modafinil, a widely prescribed wake-promoting agent used to treat several sleep-related disorders including excessive daytime sleepiness associated with narcolepsy, shift work sleep disorder, and obstructive sleep apnea/hypopnea syndrome. Remarkably, however, the neuronal circuitry through which modafinil exerts its wake-promoting effects remains unresolved. In the present study, we sought to determine if the wake-promoting effects of armodafinil are mediated, at least in part, by inhibiting the sleep-promoting neurons of the ventrolateral preoptic (VLPO) nucleus. To do so, we measured changes in waking following intraperitoneal administration of armodafinil (200 mg/kg) or the psychostimulant methamphetamine (1 mg/kg) in rats with cell-body specific lesion of the VLPO. Rats with histologically confirmed lesions of the VLPO demonstrated a sustained increase in wakefulness at baseline, but the increase in wakefulness following administration of both armodafinil and methamphetamine was similar to that of intact animals. These data suggest that armodafinil increases wakefulness by mechanisms that extend beyond inhibition of VLPO neurons.
  • Thumbnail Image
    Publication
    A translational approach to capture gait signatures of neurological disorders in mice and humans
    (Nature Publishing Group UK, 2017) Broom, Lauren; Ellison, Brian A.; Worley, Audrey; Wagenaar, Lara; Sörberg, Elina; Ashton, Christine; Bennett, David A.; Buchman, Aron S.; Saper, Clifford; Shih, Ludy; Hausdorff, Jeffrey M.; VanderHorst, Veronique G.
    A method for capturing gait signatures in neurological conditions that allows comparison of human gait with animal models would be of great value in translational research. However, the velocity dependence of gait parameters and differences between quadruped and biped gait have made this comparison challenging. Here we present an approach that accounts for changes in velocity during walking and allows for translation across species. In mice, we represented spatial and temporal gait parameters as a function of velocity and established regression models that reproducibly capture the signatures of these relationships during walking. In experimental parkinsonism models, regression curves representing these relationships shifted from baseline, implicating changes in gait signatures, but with marked differences between models. Gait parameters in healthy human subjects followed similar strict velocity dependent relationships which were altered in Parkinson’s patients in ways that resemble some but not all mouse models. This novel approach is suitable to quantify qualitative walking abnormalities related to CNS circuit dysfunction across species, identify appropriate animal models, and it provides important translational opportunities.
  • Thumbnail Image
    Publication
    GABAergic parafacial zone is a medullary slow–wave–sleep promoting center
    (2014) Anaclet, Christelle; Ferrari, Loris; Arrigoni, Elda; Bass, Caroline E.; Saper, Clifford; Lu, Jun; Fuller, Patrick
    Work in animals and humans suggest the existence of a slow–wave sleep (SWS) promoting/EEG synchronizing center in the mammalian lower brainstem. While sleep–active GABAergic neurons in the medullary parafacial zone (PZ) are needed for normal SWS, it remains unclear if these neurons can initiate and maintain SWS or EEG slow wave activity (SWA) in behaving mice. We used genetically targeted activation and optogenetic–based mapping to uncover the downstream circuitry engaged by SWS–promoting PZ neurons, and we show that this circuit uniquely and potently initiates SWS and EEG SWA, regardless of the time of day. PZ neurons monosynaptically innervate and release synaptic GABA onto parabrachial neurons that in turn project to and release synaptic glutamate onto cortically–projecting neurons of the magnocellular basal forebrain; hence a circuit substrate is in place through which GABAergic PZ neurons can potently trigger SWS and modulate the cortical EEG.
  • Thumbnail Image
    Publication
    A Proposal for a Coordinated Effort for the Determination of Brainwide Neuroanatomical Connectivity in Model Organisms at a Mesoscopic Scale
    (Public Library of Science, 2009) Bohland, Jason W.; Wu, Caizhi; Barbas, Helen; Bokil, Hemant; Bota, Mihail; Cline, Hollis T.; Freed, Peter J.; Greenspan, Ralph J.; Haber, Suzanne N.; Hawrylycz, Michael; Hilgetag, Claus C.; Jones, Allan; Karten, Harvey J.; Kleinfeld, David; Kötter, Rolf; Lester, Henry A.; Lin, John M.; Mikula, Shawn; Panksepp, Jaak; Price, Joseph L.; Safdieh, Joseph; Schiff, Nicholas D.; Stillman, Bruce W.; Svoboda, Karel; Swanson, Larry W.; Toga, Arthur W.; Van Essen, David C.; Mitra, Partha P.; Sporns, Olaf; Breiter, Hans C.; Doyle, John C.; Herrera, Daniel Gustavo; Huang, Z. Josh; Jones, Edward G.; Mensh, Brett Daren; Saper, Clifford; Schmahmann, Jeremy; Watson, James D.
    In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.
  • Thumbnail Image
    Publication
    The Neuroscience Peer Review Consortium
    (BioMed Central, 2009) Saper, Clifford; Maunsell, John H. R.; Sagvolden, Terje
    The Neuroscience Peer Review Consortium (NPRC) was conceived in the summer of 2007 at a meeting of editors and publishers of neuroscience journals. One of the working groups addressed whether it was possible to construct a system for permitting authors whose manuscript received supportive reviews at one journal but was not accepted to send a revised manuscript together with its first round of reviews to a new journal for the second round. This would speed up the review process and reduce the work for reviewers and editors. The working group not only designed a framework for transferring reviews among journals, but also implemented it as the NPRC. By the fall of 2007, more than a dozen major journals had signed onto the NPRC, sufficient to launch the experiment in January, 2008. We invite authors who have not yet used the NPRC to try this method for appropriate manuscripts. In order to encourage dissemination of the details outlined in this Editorial, it will also be published in other journals in the Neuroscience Peer Review Consortium.
  • Thumbnail Image
    Publication
    The Neuroscience Peer Review Consortium
    (BioMed Central, 2009) Saper, Clifford; Maunsell, John H. R.
    As the Neuroscience Peer Review Consortium (NPRC) ends its first year, it is worth looking back to see how the experiment has worked. In order to encourage dissemination of the details outlined in this Editorial, it will also be published in other journals in the Neuroscience Peer Review Consortium.
  • Thumbnail Image
    Publication
    Supramammillary glutamate neurons are a key node of the arousal system
    (Nature Publishing Group UK, 2017) Pedersen, Nigel P.; Ferrari, Loris; Venner, Anne; Wang, Joshua L.; Abbott, Stephen B. G.; Vujovic, Nina; Arrigoni, Elda; Saper, Clifford; Fuller, Patrick
    Basic and clinical observations suggest that the caudal hypothalamus comprises a key node of the ascending arousal system, but the cell types underlying this are not fully understood. Here we report that glutamate-releasing neurons of the supramammillary region (SuMvglut2) produce sustained behavioral and EEG arousal when chemogenetically activated. This effect is nearly abolished following selective genetic disruption of glutamate release from SuMvglut2 neurons. Inhibition of SuMvglut2 neurons decreases and fragments wake, also suppressing theta and gamma frequency EEG activity. SuMvglut2 neurons include a subpopulation containing both glutamate and GABA (SuMvgat/vglut2) and another also expressing nitric oxide synthase (SuMNos1/Vglut2). Activation of SuMvgat/vglut2 neurons produces minimal wake and optogenetic stimulation of SuMvgat/vglut2 terminals elicits monosynaptic release of both glutamate and GABA onto dentate granule cells. Activation of SuMNos1/Vglut2 neurons potently drives wakefulness, whereas inhibition reduces REM sleep theta activity. These results identify SuMvglut2 neurons as a key node of the wake−sleep regulatory system.