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Pietilainen, Olli

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Pietilainen

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Olli

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Pietilainen, Olli

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2018 - 20202

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Author's Publications: search.filters.isAuthorOfPublication.7b453af8-4d49-4e25-865e-6cb6163e38b7

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    Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission
    (2018) Nehme, Ralda; Zuccaro, Emanuela; Dia Ghosh, Sulagna; Li, Chenchen; Sherwood, John; Pietilainen, Olli; Barrett, Lindy; Limone, Francesco; Worringer, Kathleen A.; Kommineni, Sravya; Zang, Ying; Cacchiarelli, Davide; Meissner, Alex; Adolfsson, Rolf; Haggarty, Stephen; Madison, Jon; Muller, Matthias; Arlotta, Paola; Fu, Zhanyan; Feng, Guoping; Eggan, Kevin
    SUMMARY Transcription factor programming of pluripotent stem cells (PSCs) has emerged as an approach to generate human neurons for disease modeling. However, programming schemes produce a variety of cell types, and those neurons that are made often retain an immature phenotype, which limits their utility in modeling neuronal processes, including synaptic transmission. We report that combining NGN2 programming with SMAD and WNT inhibition generates human patterned induced neurons (hpiNs). Single-cell analyses showed that hpiN cultures contained cells along a developmental continuum, ranging from poorly differentiated neuronal progenitors to well-differentiated, excitatory glutamatergic neurons. The most differentiated neurons could be identified using a CAMK2A::GFP reporter gene and exhibited greater functionality, including NMDAR-mediated synaptic transmission. We conclude that utilizing single-cell and reporter gene approaches for selecting successfully programmed cells for study will greatly enhance the utility of hpiNs and other programmed neuronal populations in the modeling of nervous system disorders.
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    C9orf72 suppresses systemic and neural inflammation induced by gut bacteria
    (Springer Science and Business Media LLC, 2020-05-13) Burberry, Aaron; Wells, Michael; Limone, Francesco; Couto, Alexander; Smith, Kevin; Van Gastel, Nick; Wang, Jin-Yuan; Pietilainen, Olli; Qian, Menglu; Cantrell, Chris; Mok, Woon Jong Joanie; Scadden, David; Eggan, Kevin
    A hexanucleotide repeat expansion in C9ORF72 is the most common genetic variant contributing to Amyotrophic lateral sclerosis (ALS) and Frontotemporal dementia (FTD)1,2. The C9ORF72 mutation acts through gain and loss of function mechanisms to induce pathways implicated in neural degeneration3–9. The expansion is transcribed into a long repetitive RNA, which may negatively sequester RNA binding proteins4 prior to its non-canonical translation into neural-toxic di-peptide proteins3,5. Failure of RNA-polymerase to read through the mutation also reduces abundance of the endogenous C9ORF72 gene product, which functions in endo-lysosomal pathways and suppresses systemic and neural inflammation6–9. Notably, effects of the repeat expansion act with incomplete penetrance in ALS/FTD families, indicating that either genetic or environmental factors modify each individual’s risk of disease. Identifying disease modifiers is of significant translational interest, as it could suggest strategies that diminish the risk of developing ALS/FTD, or that slow progression. Here, we report that an environment with reduced abundance of immune-stimulating bacteria10,11 protects C9orf72 mutant mice from premature mortality and significantly ameliorates their underlying systemic inflammation and autoimmunity. Consistent with C9orf72 functioning to prevent microbiota from inducing a pathological inflammatory response, we found that reducing microbial burden in mutants with broad spectrum antibiotics, as well as transplanting gut microflora from a protective environment attenuated inflammatory phenotypes, even after their onset. Our studies provide further evidence that the microbial constituency of our gut plays an important role in brain health and can interact in surprising ways with well-known genetic risk factors for nervous system disorders.