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Perlman, Or

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Perlman

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Perlman, Or
Author's Publications: search.filters.isAuthorOfPublication.7c9a3ff4-cf36-4a29-8f08-9e339742c6a8

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    Pulseq‐CEST: Towards multi‐site multi‐vendor compatibility and reproducibility of CEST experiments using an open‐source sequence standard
    (Wiley, 2021-05-07) Herz, Kai; Mueller, Sebastian; Perlman, Or; Zaitsev, Maxim; Knutsson, Linda; Sun, Phillip Zhe; Zhou, Jinyuan; van Zijl, Peter; Heinecke, Kerstin; Schuenke, Patrick; Farrar, Christian; Schmidt, Manuel; Dörfler, Arnd; Scheffler, Klaus; Zaiss, Moritz
    Purpose: As the field of CEST grows, various novel preparation periods using different parameters are being introduced. At the same time, large, multisite clinical studies require clearly defined protocols, especially across different vendors. Here, we propose a CEST definition standard using the open Pulseq format for a shareable, simple, and exact definition of CEST protocols. Methods: We present the benefits of such a standard in three ways: (1) an open database on GitHub, where fully defined, human-­readable CEST protocols can be shared; (2) an open-­source Bloch-­McConnell simulation to test and optimize CEST preparation periods in silico; and (3) a hybrid MR sequence that plays out the CEST preparation period and can be combined with any existing readout module. Results: The exact definition of the CEST preparation period, in combination with the flexible simulation, leads to a good match between simulations and measurements. The standard allowed finding consensus on three amide proton transfer–­weighted protocols that could be compared in healthy subjects and a tumor patient. In addition, we could show coherent multisite results for a sophisticated CEST method, highlighting the benefits regarding protocol sharing and reproducibility. Conclusion: With Pulseq-­ CEST, we provide a straightforward approach to standardize, share, simulate, and measure different CEST preparation schemes, which are inherently completely defined.
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    Redesigned Reporter Gene for Improved Proton Exchange-Based Molecular MRI Contrast
    (Springer Science and Business Media LLC, 2020-11-26) Perlman, Or; Ito, Hirotaka; Gilad, Assaf A.; McMahon, Michael T.; Chiocca, E.; Nakashima, Hiroshi; Farrar, Christian
    Reporter gene imaging allows for non-invasive monitoring of molecular processes in living cells, providing insights on the mechanisms underlying pathology and therapy. A lysine-rich protein (LRP) chemical exchange saturation transfer (CEST) MRI reporter gene has previously been developed and used to image tumor cells, cardiac viral gene transfer, and oncolytic virotherapy. However, the highly repetitive nature of the LRP reporter gene sequence leads to DNA recombination events and the expression of a range of truncated LRP protein fragments, thereby greatly limiting the CEST sensitivity. Here we report the use of a redesigned LRP reporter (rdLRP), aimed to provide excellent stability and CEST sensitivity. The rdLRP contains no DNA repeats or GC rich regions and 30% less positively charged amino-acids. RT-PCR of cell lysates transfected with rdLRP demonstrated a stable reporter gene with a single distinct band corresponding to full-length DNA. A distinct increase in CEST-MRI contrast was obtained in cell lysates of rdLRP transfected cells and in in vivo LRP expressing mouse brain tumors ( p = 0.0275 , n = 10).
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    MR fingerprinting for semisolid magnetization transfer and chemical exchange saturation transfer quantification
    (Wiley, 2022-03-03) Perlman, Or; Farrar, Christian; Heo, Hye‐Young