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Ottoboni, Linda

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Ottoboni

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Linda

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Ottoboni, Linda
Author's Publications: search.filters.isAuthorOfPublication.8b6c22a8-19e4-4f5a-8b25-41445b65d7c5

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    CD33 Alzheimer’s disease locus: Altered monocyte function and amyloid biology
    (2013) Bradshaw, Elizabeth M.; Chibnik, Lori; Keenan, Brendan T; Ottoboni, Linda; Raj, Towfique; Tang, Anna; Rosenkrantz, Laura L; Imboywa, Selina; Lee, Michelle Ann; Von Korff, Alina; Morris, Martha C; Evans, Denis A; Johnson, Keith; Sperling, Reisa; Schneider, Julie A; Bennett, David A; De Jager, Philip
    In our functional dissection of the CD33 Alzheimer’s disease susceptibility locus, we find that the rs3865444C risk allele is associated with greater cell surface expression of CD33 in monocytes (t50 = 10.06, pjoint=1.3×10–13) of young and older individuals. It is also associated with (1) diminished internalization of Aβ42) (2) accumulation of neuritic amyloid pathology and fibrillar amyloid on in vivo imaging and (3), increased numbers of activated human microglia.
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    Multicolored Stain-Free Histopathology with Coherent Raman Imaging
    (Nature Publishing Group, 2012) Xie, Xiaoliang; Freudiger, Christian Wilhelm; Orringer, Daniel A.; Saar, Brian G.; Ji, Minbiao; Zeng, Qing; Ottoboni, Linda; Ying, Wei; Waeber, Christian; Sims, John R.; De Jager, Philip; Sagher, Oren; Philbert, Martin A.; Xu, Xiaoyin; Kesari, Santosh; Young, Geoffrey; Pfannl, Rolf
    Conventional histopathology with hematoxylin & eosin (H&E) has been the gold standard for histopathological diagnosis of a wide range of diseases. However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system. Here we describe the development of an alternative, multicolored way to visualize tissue in real-time through the use of coherent Raman imaging (CRI), without the use of dyes. CRI relies on intrinsic chemical contrast based on vibrational properties of molecules and intrinsic optical sectioning by nonlinear excitation. We demonstrate that multicolor images originating from \(CH_2\) and \(CH_3\) vibrations of lipids and protein, as well as two-photon absorption of hemoglobin, can be obtained with subcellular resolution from fresh tissue. These stain-free histopathological images show resolutions similar to those obtained by conventional techniques, but do not require tissue fixation, sectioning or staining of the tissue analyzed.