Person:
Richmond, Camilla

Profile Picture

Email Address

AA Acceptance Date

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Richmond

First Name

Camilla

Name

Richmond, Camilla
Author's Publications: search.filters.isAuthorOfPublication.aa30f05b-c25b-4991-91c7-0e9e54ca4a69

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    JAK/STAT-1 Signaling Is Required for Reserve Intestinal Stem Cell Activation during Intestinal Regeneration Following Acute Inflammation
    (Elsevier, 2017) Richmond, Camilla; Rickner, Hannah; Shah, Manasvi S.; Ediger, Tracy; Deary, Luke; Zhou, Fanny; Tovaglieri, Alessio; Carlone, Diana; Breault, David
    Summary The intestinal epithelium serves as an essential barrier to the outside world and is maintained by functionally distinct populations of rapidly cycling intestinal stem cells (CBC ISCs) and slowly cycling, reserve ISCs (r-ISCs). Because disruptions in the epithelial barrier can result from pathological activation of the immune system, we sought to investigate the impact of inflammation on ISC behavior during the regenerative response. In a murine model of αCD3 antibody-induced small-intestinal inflammation, r-ISCs proved highly resistant to injury, while CBC ISCs underwent apoptosis. Moreover, r-ISCs were induced to proliferate and functionally contribute to intestinal regeneration. Further analysis revealed that the inflammatory cytokines interferon gamma and tumor necrosis factor alpha led to r-ISC activation in enteroid culture, which could be blocked by the JAK/STAT inhibitor, tofacitinib. These results highlight an important role for r-ISCs in response to acute intestinal inflammation and show that JAK/STAT-1 signaling is required for the r-ISC regenerative response.
  • Thumbnail Image
    Publication
    Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
    (Nature Publishing Group UK, 2018) Kasendra, Magdalena; Tovaglieri, Alessio; Sontheimer-Phelps, Alexandra; Jalili-Firoozinezhad, Sasan; Bein, Amir; Chalkiadaki, Angeliki; Scholl, William; Zhang, Cheng; Rickner, Hannah; Richmond, Camilla; Li, Hu; Breault, David; Ingber, Donald
    Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation. In the Intestine Chip, the epithelium forms villi-like projections lined by polarized epithelial cells that undergo multi-lineage differentiation similar to that of intestinal organoids, however, these cells expose their apical surfaces to an open lumen and interface with endothelium. Transcriptomic analysis also indicates that the Intestine Chip more closely mimics whole human duodenum in vivo when compared to the duodenal organoids used to create the chips. Because fluids flowing through the lumen of the Intestine Chip can be collected continuously, sequential analysis of fluid samples can be used to quantify nutrient digestion, mucus secretion and establishment of intestinal barrier function over a period of multiple days in vitro. The Intestine Chip therefore may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.