Person:
Yang, Wenlong

Profile Picture

Email Address

AA Acceptance Date

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Yang

First Name

Wenlong

Name

Yang, Wenlong

Filters

2014 - 20162

Settings

Author's Publications: search.filters.isAuthorOfPublication.aeee8d5c-ebfc-444a-91af-3bfc58b4ffd6

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Stimulated Raman Scattering Microscopy with a Robust Fibre Laser Source
    (Nature Publishing Group, 2014) Freudiger, Christian Wilhelm; Yang, Wenlong; Holtom, Gary; Peyghambarian, Nasser; Xie, Xiaoliang; Kieu, Khanh Q.
    Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame \(s^{−1}\).
  • Thumbnail Image
    Publication
    Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging
    (Nature Publishing Group, 2016) Tian, Feng; Yang, Wenlong; Mordes, Daniel; Wang, Jin-Yuan; Salameh, Johnny S.; Mok, Woon Jong Joanie; Chew, Jeannie; Sharma, Aarti; Leno-Duran, Ester; Suzuki-Uematsu, Satomi; Suzuki, Naoki; Han, Steve S.; Lu, Fa-Ke; Ji, Minbiao; Zhang, Rosanna; Liu, Yue; Strominger, Jack; Shneider, Neil A.; Petrucelli, Leonard; Xie, X. Sunney; Eggan, Kevin
    The study of amyotrophic lateral sclerosis (ALS) and potential interventions would be facilitated if motor axon degeneration could be more readily visualized. Here we demonstrate that stimulated Raman scattering (SRS) microscopy could be used to sensitively monitor peripheral nerve degeneration in ALS mouse models and ALS autopsy materials. Three-dimensional imaging of pre-symptomatic SOD1 mouse models and data processing by a correlation-based algorithm revealed that significant degeneration of peripheral nerves could be detected coincidentally with the earliest detectable signs of muscle denervation and preceded physiologically measurable motor function decline. We also found that peripheral degeneration was an early event in FUS as well as C9ORF72 repeat expansion models of ALS, and that serial imaging allowed long-term observation of disease progression and drug effects in living animals. Our study demonstrates that SRS imaging is a sensitive and quantitative means of measuring disease progression, greatly facilitating future studies of disease mechanisms and candidate therapeutics.