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Tzur, Amit

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Tzur

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Amit

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Tzur, Amit

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2012 - 20132

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Author's Publications: search.filters.isAuthorOfPublication.af506f28-9100-40ea-a57a-2840746cbc24

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    Gas2l3, a Novel Constriction Site-Associated Protein Whose Regulation Is Mediated by the \(APC/C^{Cdh1}\) Complex
    (Public Library of Science, 2013) Pe’er, Tal; Lahmi, Roxane; Sharaby, Yaara; Chorni, Evelin; Noach, Meirav; Vecsler, Manuela; Zlotorynski, Eitan; Steen, Hanno; Steen, Judith A.; Tzur, Amit
    Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the \(APC/C^{Cdh1}\) complex, but not by the \(APC/C^{Cdc20}\) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.
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    Direct observation of mammalian cell growth and size regulation
    (2012) Son, Sungmin; Tzur, Amit; Weng, Yaochung; Jorgensen, Paul; Kim, Jisoo; Kirschner, Marc; Manalis, Scott R.
    We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1/S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.