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Niu, Jia

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Niu

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Jia

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Niu, Jia

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2012 - 20142

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Author's Publications: search.filters.isAuthorOfPublication.b352f69b-7c86-4602-8697-a03b02e5b6af

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    Translation of DNA into Evolvable Sequence-Defined Synthetic Polymers
    (2014-06-06) Niu, Jia; Liu, David Ruchien; Szostak, Jack; Saghatelian, Alan
    Laboratory directed evolution have enabled the discovery of numerous functional natural and synthetic macromolecules with tailor-made functions. However, approaches that use enzymes to effect the crucial translation from an information carrier molecule such as DNA or RNA to synthetic polymers are limited to producing close analogs of nucleic acids, either due to a strict requirement to hybridize with a nucleic acid template or as a consequence of the limited substrate scope of polymerase enzymes.
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    DNA Ligase-Mediated Translation of DNA Into Densely Functionalized Nucleic Acid Polymers
    (American Chemical Society, 2012) Hili, Ryan; Niu, Jia; Liu, David
    We developed a method to translate DNA sequences into densely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization of 5′-phosphorylated trinucleotides containing a wide variety of appended functional groups. This polymerization proceeds sequence specifically along a DNA template and can generate polymers of at least 50 building blocks (150 nucleotides) in length with remarkable efficiency. The resulting single-stranded highly modified nucleic acid is a suitable template for primer extension using deep vent (exo-) DNA polymerase, thereby enabling the regeneration of template DNA. We integrated these capabilities to perform iterated cycles of in vitro translation, selection, and template regeneration on libraries of modified nucleic acid polymers.