Person: Joyce, Eric F.
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Publication Oligopaints: highly efficient, bioinformatically designed probes for fluorescence in situ hybridization
(BioMed Central, 2013) Beliveau, Brian; Joyce, Eric F.; Apostolopoulosa, Nicholas; Yilmaza, Feyza; Fonseka, Chamith Y; McCole, Ruth; Chang, Yiming; Li, Jin Billy; Senaratne, Niroshi Niroshini; Williams, Benjamin R; Rouillard, Jean-Marie; Wu, Chao-tingPublication Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes
(Nature Pub. Group, 2015) Beliveau, Brian; Boettiger, Alistair; Avendaño, Maier S.; Jungmann, Ralf; McCole, Ruth; Joyce, Eric F.; Kim-Kiselak, Caroline; Bantignies, Frédéric; Fonseka, Chamith; Erceg, Jelena; Hannan, Mohammed; Hoang, Hien G.; Colognori, David; Lee, Jeannie; Shih, William; Yin, Peng; Zhuang, Xiaowei; Wu, Chao-tingFluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis.