(Nature Publishing Group, 2009) Chen, Y. Grace; Kowtoniuk, Walter Eugene; Agarwal, Isha; Shen, Yinghua; Liu, David
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs, and is present at a surprisingly high abundance of ~3000 copies per cell.
