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Lee, Ben

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Lee

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Ben

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Lee, Ben
Author's Publications: search.filters.isAuthorOfPublication.c7d27c03-29ba-49d6-a3a2-8d8fbb449419

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    Production of Mucosally Transmissible SHIV Challenge Stocks from HIV-1 Circulating Recombinant Form 01_AE env Sequences
    (Public Library of Science, 2016) Tartaglia, Lawrence; Chang, Hui-Wen; Lee, Ben; Abbink, Peter; Ng’ang’a, David; Boyd, Michael; Lavine, Christy; Lim, So-Yon; Sanisetty, Srisowmya; Whitney, James; Seaman, Michael; Rolland, Morgane; Tovanabutra, Sodsai; Ananworanich, Jintanat; Robb, Merlin L.; Kim, Jerome H.; Michael, Nelson L.; Barouch, Dan
    Simian-human immunodeficiency virus (SHIV) challenge stocks are critical for preclinical testing of vaccines, antibodies, and other interventions aimed to prevent HIV-1. A major unmet need for the field has been the lack of a SHIV challenge stock expressing circulating recombinant form 01_AE (CRF01_AE) env sequences. We therefore sought to develop mucosally transmissible SHIV challenge stocks containing HIV-1 CRF01_AE env derived from acutely HIV-1 infected individuals from Thailand. SHIV-AE6, SHIV-AE6RM, and SHIV-AE16 contained env sequences that were >99% identical to the original HIV-1 isolate and did not require in vivo passaging. These viruses exhibited CCR5 tropism and displayed a tier 2 neutralization phenotype. These challenge stocks efficiently infected rhesus monkeys by the intrarectal route, replicated to high levels during acute infection, and established chronic viremia in a subset of animals. SHIV-AE16 was titrated for use in single, high dose as well as repetitive, low dose intrarectal challenge studies. These SHIV challenge stocks should facilitate the preclinical evaluation of vaccines, monoclonal antibodies, and other interventions targeted at preventing HIV-1 CRF01_AE infection.
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    Buckling of Elastomeric Beams Enables Actuation of Soft Machines
    (Wiley-Blackwell, 2015) Yang, Dian; Mosadegh, Bobak; Ainla, Alar; Lee, Ben; Khashai, Fatemeh; Suo, Zhigang; Bertoldi, Katia; Whitesides, George
    Soft, pneumatic actuators that buckle when interior pressure is less than exterior provide a new mechanism of actuation. Upon application of negative pneumatic pressure, elastic beam elements in these actuators undergo reversible, cooperative collapse, and generate a rotational motion. These actuators are inexpensive to fabricate, lightweight, easy to control, and safe to operate. They can be used in devices that manipulate objects, locomote, or interact cooperatively with humans.