Person:
Haynes, Karmella A.

Profile Picture

Email Address

AA Acceptance Date

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Haynes

First Name

Karmella A.

Name

Haynes, Karmella A.
Author's Publications: search.filters.isAuthorOfPublication.da531156-6a42-49f3-9dc1-784bf4a1e529

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    A BioBrick compatible strategy for genetic modification of plants
    (BioMed Central, 2012) Boyle, Patrick M; Burrill, Devin Rene; Inniss, Mara Christine; Agapakis, Christina M; Deardon, Aaron; dewerd, Jonathan; Gedeon, Michael A; Quinn, Jacqueline Y; Paull, Morgan L; Raman, Anugraha M; Theilmann, Mark R; Wang, Lu; Winn, Julia C; Medvedik, Oliver; Schellenberg, Kurt William; Haynes, Karmella A.; Viel, Alain; Brenner, Tamara; Church, George; Shah, Jagesh; Silver, Pamela
    Background: Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process. Results: This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (http://www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin. Conclusions: Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.
  • Thumbnail Image
    Publication
    Eukaryotic Systems Broaden the Scope of Synthetic Biology
    (The Rockefeller University Press, 2009) Haynes, Karmella A.; Silver, Pamela
    Synthetic biology aims to engineer novel cellular functions by assembling well-characterized molecular parts (i.e., nucleic acids and proteins) into biological “devices” that exhibit predictable behavior. Recently, efforts in eukaryotic synthetic biology have sprung from foundational work in bacteria. Designing synthetic circuits to operate reliably in the context of differentiating and morphologically complex cells presents unique challenges and opportunities for progress in the field. This review surveys recent advances in eukaryotic synthetic biology and describes how synthetic systems can be linked to natural cellular processes in order to manipulate cell behavior and to foster new discoveries in cell biology research.