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Jiang, Lan

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Jiang

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Jiang, Lan
Author's Publications: search.filters.isAuthorOfPublication.ebdea551-6dd7-40b9-a016-1057a2e0e191

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Now showing 1 - 4 of 4
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    Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity
    (Elsevier BV, 2017) Chen, Renchao; Wu, Xiaoji; Jiang, Lan; Zhang, Yi
    Chen et al. perform single-cell RNA sequencing analysis of the adult mouse hypothalamus to probe the rich cell diversity of this complex brain region. They also identify neuronal subtypespecific transcriptional responses to food deprivation.
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    Single-Cell Transcript Profiles Reveal Multilineage Priming in Early Progenitors Derived from Lgr5 + Intestinal Stem Cells
    (Elsevier BV, 2016-08) Kim, Tae-Hee; Saadatpour, Assieh; Guo, Guoji; Saxena, Madhurima; Cavazza, Alessia; Desai, Niyati; Jadhav, Unmesh; Jiang, Lan; Rivera, Miguel; Orkin, Stuart; Yuan, Guo-Cheng; Shivdasani, Ramesh
    Lgr5+ intestinal stem cells (ISC) drive epithelial self-renewal, and their immediate progeny – intestinal bipotential progenitors – produce absorptive and secretory lineages via lateral inhibition. To define features of early transit from the ISC compartment, we used a microfluidics approach to measure selected stem- and lineage-specific transcripts in single Lgr5+ cells. We identified two distinct cell populations, one that expresses known ISC markers and a second, abundant population that simultaneously expresses markers of stem and mature absorptive and secretory cells. Single-molecule mRNA in situ hybridization and immunofluorescence verified expression of lineage-restricted genes in a subset of Lgr5+ cells in vivo. Transcriptional network analysis revealed that one group of Lgr5+ cells arises from the other and displays characteristics expected of bipotential progenitors, including activation of Notch ligand and cell-cycle inhibitor genes. These findings define the earliest steps in ISC differentiation and reveal multilineage gene priming as a fundamental property of the process.
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    Genomic imprinting of Xist by maternal H3K27me3
    (Cold Spring Harbor Laboratory, 2017-10-01) Inoue, Azusa; Jiang, Lan; Lu, Falong; Zhang, Yi
    Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte growth and persists through preimplantation development in mice. Loss of maternal H3K27me3 induces maternal Xist expression and maternal XCI in preimplantation embryos. Our study thus identifies maternal H3K27me3 as the imprinting mark of Xist.
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    Role of Tet proteins in enhancer activity and telomere elongation
    (Cold Spring Harbor Laboratory Press, 2014) Lu, Falong; Liu, Yuting; Jiang, Lan; Yamaguchi, Shinpei; Zhang, Yi
    DNA methylation at the C-5 position of cytosine (5mC) is one of the best-studied epigenetic modifications and plays important roles in diverse biological processes. Iterative oxidation of 5mC by the ten-eleven translocation (Tet) family of proteins generates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC are selectively recognized and excised by thymine DNA glycosylase (TDG), leading to DNA demethylation. Functional characterization of Tet proteins has been complicated by the redundancy between the three family members. Using CRISPR/Cas9 technology, we generated mouse embryonic stem cells (ESCs) deficient for all three Tet proteins (Tet triple knockout [TKO]). Whole-genome bisulfite sequencing (WGBS) analysis revealed that Tet-mediated DNA demethylation mainly occurs at distally located enhancers and fine-tunes the transcription of genes associated with these regions. Functional characterization of Tet TKO ESCs revealed a role for Tet proteins in regulating the two-cell embryo (2C)-like state under ESC culture conditions. In addition, Tet TKO ESCs exhibited increased telomere–sister chromatid exchange and elongated telomeres. Collectively, our study reveals a role for Tet proteins in not only DNA demethylation at enhancers but also regulating the 2C-like state and telomere homeostasis.