Person:
Gao, Fan

Profile Picture

Email Address

AA Acceptance Date

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Gao

First Name

Fan

Name

Gao, Fan

Filters

20191

Settings

Author's Publications: search.filters.isAuthorOfPublication.fc17803a-1c95-45a2-ae91-38e132a520f5

Search Results

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Publication
    Single-Cell Transcriptomic Analysis of Alzheimer’s Disease
    (Springer Science and Business Media LLC, 2019-05-01) Gao, Fan; Kellis, Manolis; Mathys, Hansruedi; Davila-Velderrain, Jose; Peng, Zhuyu; Mohammadi, Shahin; Young, Jennie; Menon, Madhvi; He, Liang; Abdurrob, Fatema; Jiang, Xueqiao; Martorell, Anthony; Ransohoff, Richard; Hafler, Brian; Bennett, David; Tsai, Li-Huei
    Alzheimer’s disease (AD) is a pervasive neurodegenerative disorder whose molecular and cellular complexity remains poorly understood. Here, we profiled and analyzed 80,660 single-nucleus transcriptomes from prefrontal cortex of 48 individuals with varying degrees of AD pathology. We identified transcriptionally-distinct subpopulations across six major brain cell-types, including those associated with pathology and characterized by regulators of myelination, inflammation, and neuron survival. The strongest AD-associated changes appeared early in pathological progression and were highly cell type-specific, whereas genes upregulated in late-stage were common across cell types and primarily involved in global stress response. Surprisingly, we found an overrepresentation of female cells in AD-associated subpopulations, and substantially different transcriptional responses between sexes in multiple cell types including oligodendrocytes. Overall, myelination-related processes were recurrently perturbed in multiple cell types, suggesting a key role in AD pathophysiology. Our single-cell transcriptomic resource provides a first blueprint for interrogating the molecular underpinnings and cellular basis of AD.