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Pishchany, Glib

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Pishchany

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Glib

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Pishchany, Glib

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2016 - 20182

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Author's Publications: search.filters.isAuthorOfPublication.fc887b3d-2955-4f74-9c75-4a87385bf011

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    Stingless Bee Larvae Require Fungal Steroid to Pupate
    (Nature Publishing Group UK, 2018) Paludo, Camila R.; Menezes, Cristiano; Silva-Junior, Eduardo A.; Vollet-Neto, Ayrton; Andrade-Dominguez, Andres; Pishchany, Glib; Khadempour, Lily; do Nascimento, Fabio S.; Currie, Cameron R.; Kolter, Roberto; Clardy, Jon; Pupo, Mônica T.
    The larval stage of the stingless bee Scaptotrigona depilis must consume a specific brood cell fungus in order to continue development. Here we show that this fungus is a member of the genus Zygosaccharomyces and provides essential steroid precursors to the developing bee. Insect pupation requires ecdysteroid hormones, and as insects cannot synthesize sterols de novo, they must obtain steroids in their diet. Larval in vitro culturing assays demonstrated that consuming ergosterol recapitulates the developmental effects on S. depilis as ingestion of Zygosaccharomyces sp. cells. Thus, we determined the molecular underpinning of this intimate mutualistic symbiosis. Phylogenetic analyses showed that similar cases of bee-Zygosaccharomyces symbiosis may exist. This unprecedented case of bee-fungus symbiosis driven by steroid requirement brings new perspectives regarding pollinator-microbiota interaction and preservation.
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    Whole-Genome Sequence of Bacillus sp. SDLI1, Isolated from the Social Bee Scaptotrigona depilis
    (American Society for Microbiology, 2016) Paludo, Camila R.; Ruzzini, Antonio C.; Silva-Junior, Eduardo A.; Pishchany, Glib; Currie, Cameron R.; Nascimento, Fábio S.; Kolter, Roberto; Clardy, Jon; Pupo, Mônica T.
    We announce the complete genome sequence of Bacillus sp. strain SDLI1, isolated from larval gut of the stingless bee Scaptotrigona depilis. The 4.13-Mb circular chromosome harbors biosynthetic gene clusters for the production of antimicrobial compounds.