Publication: Efficient proximity labeling in living cells and organisms with TurboID
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Date
2018-10
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Springer Science and Business Media LLC
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Citation
Branon, Tess C, Justin Bosch, Ariana D Sanchez, Namrata Udeshi, Tanya Svinkina, Steven A. Carr, Jessica L Feldman et al. "Efficient proximity labeling in living cells and organisms with TurboID." Nature Biotechnology 36, no. 9 (2018): 880-887. DOI: 10.1038/nbt.4201
Research Data
Abstract
Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 hour labeling times or utilize chemicals with limited cell permeability or high toxicity. We used yeast display-based directed evolution to engineer two promiscuous mutants of biotin ligase, TurboID and miniTurbo, which catalyze PL with much greater efficiency than BioID or BioID2, and enable 10-minute PL in cells with non-toxic and easily deliverable biotin. Furthermore, TurboID extends biotin-based PL to flies and worms.
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Keywords
Biomedical Engineering, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology
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