Technologies for Multiplexed High Throughput Screens

Abstract

Biological processes are often far too complex to predict. For those phenomenon that still evade understanding, it is helpful to visualize a black box—a system with clear inputs and outputs, but an unknowable, labyrinthine set of interior mechanisms to produce this transformation. To determine what happened, controlled experiments are often run on a culture of cells or tissues using a multi-well plate format. This structure is relatively simple and can be applied in most laboratory environments. This paper discusses newly built technologies that take this definition of an experiment and adapt it to detect genotypes as well as phenotypes; promisingly, these technologies are highly multiplexible by using a modified version of Fluoresecnt in situ Sequencing (FISSEQ).