Publication: Separating Stem Cells by Flow Cytometry: Reducing Variability for Solid Tissues
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Date
2009-12-04
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Elsevier BV
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Citation
Alexander, Caroline M., Joel Puchalski, Kristine S. Klos, Nisha Badders, Laurie Ailles, Carla Kim, Peter Dirks et al. "Separating Stem Cells by Flow Cytometry: Reducing Variability for Solid Tissues." Cell Stem Cell 5, no. 6 (2009): 579-583. DOI: 10.1016/j.stem.2009.11.008
Research Data
Abstract
Until there are valid identifiers that visualize stem cells in vivo, we rely upon flow cytometry to enrich for subpopulations with stem cell function. However, data reporting styles for flow cytometric analyses are typically inconsistent, creating challenges in comparing results across publications. In our view, clear reporting guidelines could improve reproducibility of stem cell analyses in solid tissues.
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Research Subject Categories::MEDICINE::Dermatology and venerology,clinical genetics, internal medicine::Internal medicine::Molecular medicine (genetics and pathology), Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics, Research Subject Categories::MEDICINE::Morphology, cell biology, pathology::Cell biology
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