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LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release

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2011

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10.1371/journal.pone.0018192

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Public Library of Science
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Lundequist, Anders, and Joshua A. Boyce. 2011. LPA5 Is abundantly expressed by human mast cells and important for lysophosphatidic acid induced MIP-1β release. PLoS ONE 6(3): e18192.

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Abstract

Background: Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings: Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions: LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065470/pdf/

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biochemistry, lipid mediators, proteins, immune system proteins, transmembrane proteins, genetics, gene expression, immunology, membrane receptor signaling, signal transduction, clinical immunology, immunologic receptor signaling, G-protein signaling

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This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service

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http://nrs.harvard.edu/urn-3:HUL.InstRepos:5146965

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