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RIC-7 Promotes Neuropeptide Secretion

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2012

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Public Library of Science
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Hao, Yingsong, Zhitao Hu, Derek Sieburth, and Joshua M. Kaplan. 2012. Ric-7 promotes neuropeptide secretion. PLoS Genetics 8(1): e1002464.

Abstract

Secretion of neurotransmitters and neuropeptides is mediated by exocytosis of distinct secretory organelles, synaptic vesicles (SVs) and dense core vesicles (DCVs) respectively. Relatively little is known about factors that differentially regulate SV and DCV secretion. Here we identify a novel protein RIC-7 that is required for neuropeptide secretion in Caenorhabditis elegans. The RIC-7 protein is expressed in all neurons and is localized to presynaptic terminals. Imaging, electrophysiology, and behavioral analysis of ric-7 mutants indicates that acetylcholine release occurs normally, while neuropeptide release is significantly decreased. These results suggest that RIC-7 promotes DCV–mediated secretion.

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