Automating Microfluidics: Reconfigurable Virtual Channels for Cell and Droplet Transport

Abstract

The emerging field of digital microfluidics promises to solve many shortcomings of traditional continuous-flow fluidics. This technology has a few incarnations, including EWOD (eletrowetting on dielectric) and DEP (dielectrophoresis) chips. Both consist of large arrays of electrical pixels which move droplets and cells. They actuate fluids actively, have error feedback, are programmable, perform operations in parallel, and do not rely on external pumps. For these reasons we foresee the increased use of digital microfluidics in the near future. We also foresee a gradual shift away from purpose-built microfluidic devices, towards multi-purpose platforms with specific applications encoded in software. To this extent we present here a new paradigm of encoding and automating microfluidic operations using video files. We use this technology to create several configurations of virtual microfluidic channels and to play film clips using living cells on a DEP chip.