Publication:

A Screen for Germination Mutants in Saccharomyces cerevisiae

Thumbnail Image

Open/View Files

3276131.pdf (1.08 MB)

Date

2011

Authors

Published Version

10.1534/g3.111.000323

Journal Title

Journal ISSN

Volume Title

Publisher

Genetics Society of America
The Harvard community has made this article openly available. Please share how this access benefits you.

Research Projects

Organizational Units

Journal Issue

Citation

Kloimwieder, Anne, and Fred Winston. 2011. A screen for germination mutants in Saccharomyces cerevisiae. G3 1(2): 143-149.

Abstract

Spore germination in Saccharomyces cerevisiae is a process in which a quiescent cell begins to divide. During germination, the cell undergoes dramatic changes in cell wall and membrane composition, as well as in gene expression. To understand germination in greater detail, we screened the S. cerevisiae deletion set for germination mutants. Our results identified two genes, TRF4 and ERG6, that are required for normal germination on solid media. TRF4 is a member of the TRAMP complex that, together with the exosome, degrades RNA polymerase II transcripts. ERG6 encodes a key step in ergosterol biosynthesis. Taken together, these results demonstrate the complex nature of germination and two genes important in the process.

Description

Other Available Sources

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276131/pdf/

Research Data

Keywords

Saccharomyces cerevisiae, germination, sporulation, ERG6, TRF4

Terms of Use

This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service

URI

http://nrs.harvard.edu/urn-3:HUL.InstRepos:10304560

Collections

HMS Scholarly Articles

Endorsement

Review

Supplemented By

Related Stories