Publication: 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus
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Date
2012
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Public Library of Science
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Citation
Lechene, Claude P., Gloria Y. Lee, J. Collin Poczatek, Mehmet Toner, and John D. Biggers. 2012. 3D multi-isotope imaging mass spectrometry reveals penetration of \(^{18}O\)-trehalose in mouse sperm nucleus. PLoS ONE 7(8): e42267.
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Abstract
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.
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Keywords
Biology, Anatomy and Physiology, Cell Physiology, Reproductive System, Biotechnology, Genetic Engineering, Transgenics, Cryobiology, Developmental Biology, Molecular Development, Histology, Model Organisms, Animal Models, Mouse
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