Publication: A Bioinformatics Method Identifies Prominent Off-targeted Transcripts in RNAi Screens
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2012
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Nature Publishing Group
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Sigoillot, Frederic D., Susan Lyman, Jeremy F. Huckins, Britt Adamson, Eunah Chung, Brian Quattrochi, and Randall W. King. 2012. A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens. Nature Methods 9(4): 363-366.
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Abstract
Because off-target effects hamper interpretation and validation of RNAi screens, we developed a bioinformatics method, Genome-wide Enrichment of Seed Sequence matches (GESS), to identify candidate off-targeted transcripts from direct analysis of primary screening data. GESS identified a prominent off-targeted transcript in several screens, including MAD2 in a screen for components of the spindle assembly checkpoint. We demonstrate how incorporation of the results of GESS analysis can enhance the validation rate in RNAi screens.
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