Publication: Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase
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Date
2013
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Nature Publishing Group
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Van Rijn, Sjoerd, Jonas Nilsson, David P. Noske, W. Peter Vandertop, Bakhos A. Tannous, and Thomas Würdinger. 2013. Functional multiplex reporter assay using tagged Gaussia luciferase. Scientific Reports 3: 1046.
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Abstract
We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc\(_{tag}\)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc\(_{tag}\) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.
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