Publication: Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy
Loading...
Open/View Files
Date
2013-10-08
Authors
Published Version
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Christodoulou, Danos C. 2013. Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy. Doctoral dissertation, Harvard University.
Abstract
Characterization of the RNA transcriptome by next-generation sequencing can produce an unprecedented yield of information that provides novel biologic insights. I describe four approaches for sequencing different aspects of the transcriptome and provide computational tools to analyze the resulting data. Methods that query the dynamic range of gene expression, low expressing transcripts, micro RNA levels, and start-site usage of transcripts are described.
Description
Other Available Sources
Research Data
Keywords
Genetics, fhl1, genetic modifier, hypertrophic cardiomyopathy, inherited cardiomyopathies, next-generation sequencing, rna-seq
Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service