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Mechanisms Governing Mesothelial Clearance by Ovarian Cancer Spheroids

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2013-10-08

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Davidowitz, Rachel Alexis. 2013. Mechanisms Governing Mesothelial Clearance by Ovarian Cancer Spheroids. Doctoral dissertation, Harvard University.

Abstract

Metastatic dissemination of ovarian tumors involves the invasion of multi-cellular tumor cell clusters into the mesothelial cell lining of organs in the peritoneal cavity. We developed an in vitro assay that models this initial step of ovarian cancer metastasis to investigate the mechanisms of invasion. Pre-clustered ovarian cancer multicellular spheroids are incubated with GFP-expressing mesothelial monolayers and the extent of mesothelial invasion is monitored by time-lapse video microscopy.

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Cellular biology, Biology, Cancer, mesothelium, Metastasis, Ovarian

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