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Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting

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2014-06-06

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Chong, Shasha. 2014. Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting. Doctoral dissertation, Harvard University.

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Advances in optical imaging techniques have allowed quantitative studies of many biological systems. This dissertation elaborates on our efforts in both developing novel imaging modalities based on detection of optical absorption and applying high-sensitivity fluorescence microscopy to the study of biology.

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Chemistry, DNA supercoiling, gyrase, optical absorption, single-molecule, transcriptional bursting

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