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Regulation of microRNA activity by translation initiation factors in melanoma

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2014-06-06

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Yanez, Adrienne Gail. 2014. Regulation of microRNA activity by translation initiation factors in melanoma. Doctoral dissertation, Harvard University.

Abstract

microRNAs (miRNAs) are small, noncoding RNAs that may regulate more than half of human genes, yet the molecular mechanism of miRNA-mediated repression remains obscure. Using a cell-free assay of miRNA activity, we show that miRNA-targeted mRNAs are enriched for components of the 40S, but not 60S ribosomal subunit. Additionally, a molecular toeprint of 18 nucleotides 3' relative to the start codon, consistent with nucleotide protection by 40S ribosomal subunits, is enriched on miRNA-targeted mRNAs. Our results suggest that miRNAs repress translation initiation in a cell-free system by preventing 60S ribosomal subunit joining to 40S subunits positioned at the start codon.

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Molecular biology, Cellular biology, eIF, microRNA, translation

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This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service

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http://nrs.harvard.edu/urn-3:HUL.InstRepos:12274340

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