Publication: Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
Loading...
Open/View Files
Date
2013
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Zhu, J., H. B. Larman, G. Gao, R. Somwar, Z. Zhang, U. Laserson, A. Ciccia, et al. 2013. “Protein interaction mapping with ribosome-displayed using PLATO ORF libraries.” Nature protocols 9 (1): 90-103. doi:10.1038/nprot.2013.167. http://dx.doi.org/10.1038/nprot.2013.167.
Abstract
Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method by identifying known and new interacting partners of LYN kinase, patient autoantibodies and the small molecules gefitinib and dasatinib.
Description
Other Available Sources
Research Data
Keywords
Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service