Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells
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Date
2014
Author(s)
Mollah, Shamim
Dobrin, Joseph
Feder, Rachel
Tse, Sze-Wah
Matos, Ines
Cheong, Cheolho
Steinman, Ralph M.
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Citation
Mollah, Shamim, Joseph Dobrin, Rachel Feder, Sze-Wah Tse, Ines Matos, Cheolho Cheong, Ralph M. Steinman, and Niroshana Anandasabapathy. 2014. “Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells.” The Journal of investigative dermatology 134 (5): 1265-1275. doi:10.1038/jid.2013.515. http://dx.doi.org/10.1038/jid.2013.515.
Abstract
Skin-derived dendritic cells (DC) are potent antigen presenting cells with critical roles in both adaptive immunity and tolerance to self. Skin DC carry antigens and constitutively migrate to the skin draining lymph nodes (LN). In mice, Langerin-CD11b− dermal DC are a low-frequency, heterogeneous, migratory DC subset that traffic to LN (Langerin-CD11b-migDC). Here, we build on the observation that Langerin-CD11b− migDC are Fms-like tyrosine kinase 3 ligand (Flt3L) dependent and strongly Flt3L responsive, which may relate them to classical DCs. Examination of DC capture of FITC from painted skin, DC isolation from skin explant culture, and from the skin of CCR7 knockout mice which accumulate migDC, demonstrate these cells are cutaneous residents. Langerin-CD11b-Flt3L responsive DC are largely CD24(+) and CX3CR1low and can be depleted from Zbtb46-DTR mice, suggesting classical DC lineage. Langerin-CD11bmigDC present antigen with equal efficiency to other DC subsets ex vivo including classical CD8α cDC and Langerin+CD103+ dermal DC. Finally, transcriptome analysis suggests a close relationship to other skin DC, and a lineage relationship to other classical DC. This work demonstrates that Langerin- CD11b− dermal DC, a previously overlooked cell subset, may be an important player in the cutaneous immune environment.
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