Publication: DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis
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2014
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Koussa, Mounir A, Ken Halvorsen, Andrew Ward, and Wesley P Wong. 2014. “DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis.” Nature methods 12 (2): 123-126. doi:10.1038/nmeth.3209. http://dx.doi.org/10.1038/nmeth.3209.
Abstract
We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states.
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DNA Origami, Nanoswitch, Multimolecular Interactions, Biomolecular Interaction Analysis, Kinetics, Affinity, Gel Electrophoresis
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