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Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation

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2017

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eLife Sciences Publications, Ltd
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Fielding, C. A., M. P. Weekes, L. V. Nobre, E. Ruckova, G. S. Wilkie, J. A. Paulo, C. Chang, et al. 2017. “Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation.” eLife 6 (1): e22206. doi:10.7554/eLife.22206. http://dx.doi.org/10.7554/eLife.22206.

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Abstract

The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation. DOI: http://dx.doi.org/10.7554/eLife.22206.001

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cytomegalovirus, human, immune evasion, natural killer cell, Virus

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