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Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials

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5231360.pdf (2.05 MB)

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2017

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10.1371/journal.pone.0169886

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Public Library of Science
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Tabish, Ali M., Katrien Poels, Hyang-Min Byun, Katrien luyts, Andrea A. Baccarelli, Johan Martens, Stef Kerkhofs, Sven Seys, Peter Hoet, and Lode Godderis. 2017. “Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials.” PLoS ONE 12 (1): e0169886. doi:10.1371/journal.pone.0169886. http://dx.doi.org/10.1371/journal.pone.0169886.

Abstract

Aims This study aimed to investigate the effects of nanomaterial (NM) exposure on DNA methylation. Methods and Results: Intra-tracheal administration of NM: gold nanoparticles (AuNPs) of 5-, 60- and 250-nm diameter; single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at high dose of 2.5 mg/kg and low dose of 0.25 mg/kg for 48 h to BALB/c mice. Study showed deregulations in immune pathways in NM-induced toxicity in vivo. NM administration had the following DNA methylation effects: AuNP 60 nm induced CpG hypermethylation in Atm, Cdk and Gsr genes and hypomethylation in Gpx; Gsr and Trp53 showed changes in methylation between low- and high-dose AuNP, 60 and 250 nm respectively, and AuNP had size effects on methylation for Trp53. Conclusion: Epigenetics may be implicated in NM-induced disease pathways.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5231360/pdf/

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Biology and life sciences, Cell biology, Chromosome biology, Chromatin, Chromatin modification, DNA methylation, Genetics, Epigenetics, Gene expression, DNA, DNA modification, Biochemistry, Nucleic acids, Engineering and Technology, Nanotechnology, Carbon Nanotubes, Biology and Life Sciences, Physical Sciences, Materials Science, Materials by Attribute, Nanomaterials, Physiology, Immune Physiology, Cytokines, Medicine and Health Sciences, Immunology, Immune System, Innate Immune System, Developmental Biology, Molecular Development, Nanoparticles, Zoology, Animal Anatomy, Vibrissae, Animal Physiology, Anatomy, Body Fluids, Blood, Hematology

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http://nrs.harvard.edu/urn-3:HUL.InstRepos:30371121

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Changes in DNA Methylation in Mouse… : DASH Story 2021-10-10
I'm using the Harvard Institutional Repository as an example while guest-teaching a freshman seminar on the importance of finding and citing scholarly sources. Thank you for your open access repository!!