Maternal obesity programs mitochondrial and lipid metabolism gene expression in infant umbilical vein endothelial cells
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Date
2016
Author(s)
Ramos Costa, Suzana Maria
Matthews, Tucker
Hughes, Katelyn
Daher, Grace
Dreyfuss, Jonathan M.
Pontes da Silva, Giselia Alves
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Citation
Ramos Costa, Suzana Maria, Elvira Isganaitis, Tucker Matthews, Katelyn Hughes, Grace Daher, Jonathan M. Dreyfuss, Giselia Alves Pontes da Silva, and Mary-Elizabeth Patti. 2016. “Maternal obesity programs mitochondrial and lipid metabolism gene expression in infant umbilical vein endothelial cells.” International journal of obesity (2005) 40 (11): 1627-1634. doi:10.1038/ijo.2016.142. http://dx.doi.org/10.1038/ijo.2016.142.
Abstract
Background/Objectives Maternal obesity increases risk for childhood obesity, but molecular mechanisms are not well understood. We hypothesized that primary umbilical vein endothelial cells (HUVEC) from infants of overweight and obese mothers would harbor transcriptional patterns reflecting offspring obesity risk. Subjects/Methods In this observational cohort study, we recruited 13 lean (pre-pregnancy BMI <25.0 kg/m2) and 24 overweight-obese (‘ov-ob’, BMI ≥25.0 kg/m2) women. We isolated primary HUVEC, and analyzed both gene expression (Primeview, Affymetrix) and cord blood levels of hormones and adipokines. Results: 142 transcripts were differentially expressed in HUVEC from infants of overweight-obese mothers (false discovery rate, FDR <0.05). Pathway analysis revealed that genes involved in mitochondrial and lipid metabolism were negatively correlated with maternal BMI (FDR <0.05). To test whether these transcriptomic patterns were associated with distinct nutrient exposures in the setting of maternal obesity, we analyzed the cord blood lipidome and noted significant increases in levels of total free fatty acids (lean: 95.5 ± 37.1 ug/ml, ov-ob: 124.1 ± 46.0 ug/ml, P=0.049), palmitate (lean: 34.5 ± 12.7 ug/ml, ov-ob: 46.3 ± 18.4 ug/ml, P=0.03) and stearate (lean: 20.8 ± 8.2 ug/ml, ov-ob: 29.7 ± 17.2 ug/ml, P=0.04), in infants of overweight-obese mothers. Conclusion: Prenatal exposure to maternal obesity alters HUVEC expression of genes involved in mitochondrial and lipid metabolism, potentially reflecting developmentally-programmed differences in oxidative and lipid metabolism.
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