Publication: CRISPR Guide RNA Design for Research Applications
No Thumbnail Available
Open/View Files
Date
2016-09
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley-Blackwell
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Mohr, Stephanie, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, Norbert Perrimon. "CRISPR Guide RNA Design for Research Applications." FEBS Journal 283, no. 17 (2016): 3232-3238. DOI: 10.1111/febs.13777
Research Data
Abstract
The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs (gRNAs). Here, we review the state of the art in CRISPR gRNA design for research applications of the CRISPR‐Cas9 system, including knockout, activation, and inhibition. Notably, achieving good gRNA design is not solely dependent on innovations in CRISPR technology. Good design and design tools also rely on availability of high‐quality genome sequence and gene annotations, as well as on availability of accumulated data regarding off‐targets and effectiveness metrics.
Description
Other Available Sources
Keywords
Research Subject Categories::TECHNOLOGY::Bioengineering::Bioinformatics, Research Subject Categories::TECHNOLOGY::Bioengineering::Genetic engineering including functional genomics