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Nevirapine Resistance in Human Immunodeficiency Virus Type 1-Positive Infants Determined Using Dried Blood Spots Stored for Up to Six Years at Room Temperature

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2009

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American Society for Microbiology
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Nelson, J. A. E., A. M. Loftis, D. Kamwendo, W. W. Fawzi, T. E. Taha, R. L. Goldenberg, and S. A. Fiscus. 2009. “Nevirapine Resistance in Human Immunodeficiency Virus Type 1-Positive Infants Determined Using Dried Blood Spots Stored for Up to Six Years at Room Temperature.” Journal of Clinical Microbiology 47 (4) (February 18): 1209–1211. doi:10.1128/jcm.00254-09.

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Dried blood spots that had been stored ambiently for 3 to 6 years lost approximately 1 log10 of human immunodeficiency virus type 1 (HIV-1) RNA, but the majority could still be genotyped for resistance. Nevirapine resistance was found in 7/16 (43.5%) HIV-1-positive HIVNET 024 infants at 4 to 6 weeks, but no resistance was found at other time points.

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